The option "Primer must span an exon-exon junction" will direct the program to return at least one primer (within a given primer pair) that spans an exon-exon junction.

Feb 4, 2025 · Primer-BLAST A tool for finding specific primers Making primers specific to your PCR template. more... Follow NCBI Connect with NLM

Primer-BLAST allows users to design new target-specific primers in one step as well as to check the specificity of pre-existing primers. Primer-BLAST also supports placing primers based on …

To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers. The longest …

Primer-BLAST was developed at NCBI to help users make primers that are specific to intended PCR target.

Despite the publication of numerous guidelines, the actual design of many published assays is often unsound: primers lack the claimed specificity, they may have to compete with secondary …

We provide a concise overview of the main primer-related issues that confront anyone wanting to design a qPCR assay, consider the main criteria that have an impact on assay performance, …

Jun 18, 2012 · We describe a robust and fully implemented general purpose primer design tool that designs target-specific PCR primers. Primer-BLAST offers flexible options to adjust the …

Sep 25, 2020 · To find primers for a group of related sequences, Primer-BLAST aligns the longest sequence to the rest to find common regions. It uses these to limit the locations of primers.

Jun 28, 2017 · The good news is that an existing tool, Primer-BLAST, fills in nicely for the functions of both Forward and Reverse e-PCR, and has the additional benefit of de novo …