Nucleosomes are barriers to RNA Polymerase II (Pol II) transcribing along gene bodies in eukaryotes. We found that a fragile “tailless nucleosome” could be generated to resolve the Pol II pausing ...
We isolated mRNA from Blumeria graminis and prepared a cDNA library using a modification of Epicentre's ExactSTART ™ Full-Length cDNA Library Cloning kit. Analysis of the 5′ ends of the cDNA by 454 ...
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