Genetic modification of mammalian cells has vast use in biotherapeutics development and basic research applications. An important step early on is the isolation of clones with the desired mutation ...
The Golden Gate method is an efficient tool for seamless assembly of multiple DNA fragments, which uses Type IIS restriction endonucleases, cleaving the DNA outside of their recognition site to ...
The existing molecular cloning methods are often limited by the availability of suitable restriction sites. It is still a challenge for simultaneous cloning of multiple fragments into different sites ...
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